Difference between revisions of "Part:BBa K2066001"

Latest revision as of 01:44, 29 October 2016

UNS Standard Adapter with Red Chromoprotein.

This is a red chromoprotein expression construct on the UNS backbone standard developed by William and Mary iGEM 2016, which includes UNS Sequences developed by Torella et. al 2013 as insert flankers that are interior to the Prefix and Suffix regions. This allows ease of cloning via Gibson Assembly or Golden Gate Assembly.

The purpose of this part is to provide a standard and visible reporter on the UNS standard, for teams to use as a template for obtaining the UNS backbone.

The UNS2 (BBa_K2066018) and UNS3 (BBa_K2066019) sequences are taken from: Torella, J. P., Boehm, C. R., Lienert, F., Chen, J. H., Way, J. C., & Silver, P. A. (2013). Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic acids research, gkt860.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 47
Illegal NheI site found at 70
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2066001 short</partinfo>
-This part serves as an adapter to the UNS standard adopted by the WM iGEM 2016 team. The part expresses a red chromoprotein as an indicator molecule and teams can perform PCR to amplify the backbone using a UNS 2 reverse and UNS 3 forward primer. This will allow you to go into a gibson assembly with another fragment containing USN 2 and 3 at either end.
+This is a red chromoprotein expression construct on the UNS backbone standard developed by William and Mary iGEM 2016, which includes UNS Sequences developed by Torella et. al 2013 as insert flankers that are interior to the Prefix and Suffix regions. This allows ease of cloning via Gibson Assembly or Golden Gate Assembly.
-The UNS2 and UNS3 sequences are taken from: Torella, J. P., Boehm, C. R., Lienert, F., Chen, J. H., Way, J. C., & Silver, P. A. (2013). Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic acids research, gkt860.
+The purpose of this part is to provide a standard and visible reporter on the UNS standard, for teams to use as a template for obtaining the UNS backbone.
+The UNS2 (BBa_K2066018) and UNS3 (BBa_K2066019) sequences are taken from: Torella, J. P., Boehm, C. R., Lienert, F., Chen, J. H., Way, J. C., & Silver, P. A. (2013). Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic acids research, gkt860.
 <!-- Add more about the biology of this part here