Difference between revisions of "Part:BBa K1915001:Experience"
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[[File:T--Georgia State--LTNF10 Western.png|frame|Figure 2: Western blot of LT10 in BL21 E. coli after Protein Induction | [[File:T--Georgia State--LTNF10 Western.png|frame|Figure 2: Western blot of LT10 in BL21 E. coli after Protein Induction | ||
| + | Ni-NTA column.]] | ||
Western blot of SDS-PAGE with His antibody | Western blot of SDS-PAGE with His antibody | ||
| − | Fraction P3 consisted of sonicated lysate. Fractions F1, Wash 1, Wash 2, Elution 1, 2, 3, and 4 from | + | Fraction P3 consisted of sonicated lysate. Fractions F1, Wash 1, Wash 2, Elution 1, 2, 3, and 4 from |
| − | + | ||
Elution 2 exhibited the strongest signal | Elution 2 exhibited the strongest signal | ||
Revision as of 01:14, 29 October 2016
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Applications of BBa_K1915001
Figure 1: Coomassie Gel Stain of LTNF10 in BL21 E. coli after Protein Induction
Coomassie gel stain of SDS-PAGE run at 100 volts for 45 minutes. Fraction P3 consisted of sonicated lysate. Fractions F1, Wash 1, Wash 2, Elution 1, 2, 3, and 4 from Ni-NTA column.
Figure 2: Western blot of LT10 in BL21 E. coli after Protein Induction Ni-NTA column.
Western blot of SDS-PAGE with His antibody Fraction P3 consisted of sonicated lysate. Fractions F1, Wash 1, Wash 2, Elution 1, 2, 3, and 4 from
Elution 2 exhibited the strongest signal
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