Difference between revisions of "Part:BBa K2100007"
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2100007 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2100007 SequenceAndFeatures</partinfo>
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Our team used hEF1a-tagBFP as a transfection marker for some of our experiments. This particular experiment characterizing the EGSH promoter measured blue fluorescent output from hEF1a-tagBFP to analyze how many copy numbers of plasmids the cells uptook. The graph shows an increase in blue fluorescence corresponds with an increase in red fluorescence at the basal level (uninduced without the coactivator), indicating that when more hEF1a-BFP plasmids are uptaken, so is the plasmid of interest, in this case pEGSH-mKate.
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https://static.igem.org/mediawiki/parts/1/17/T--MIT--EGSH.png
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This is an image of the tagBFP's blue florescence in HEK293 cells.
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https://static.igem.org/mediawiki/parts/a/a2/T--MIT--khb_bluecellsssss.jpeg
  
  

Latest revision as of 23:08, 28 October 2016


pENTR tagBFP

tagBFP is a monomeric blue fluorescent protein. We used hEF1a-tagBFP as a transfection marker in several of our experiments.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Our team used hEF1a-tagBFP as a transfection marker for some of our experiments. This particular experiment characterizing the EGSH promoter measured blue fluorescent output from hEF1a-tagBFP to analyze how many copy numbers of plasmids the cells uptook. The graph shows an increase in blue fluorescence corresponds with an increase in red fluorescence at the basal level (uninduced without the coactivator), indicating that when more hEF1a-BFP plasmids are uptaken, so is the plasmid of interest, in this case pEGSH-mKate.

T--MIT--EGSH.png

This is an image of the tagBFP's blue florescence in HEK293 cells.

T--MIT--khb_bluecellsssss.jpeg