Difference between revisions of "Part:BBa K1893015"
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<partinfo>BBa_K1893015 short</partinfo> | <partinfo>BBa_K1893015 short</partinfo> | ||
| − | A construct for controlling the expression of a gene in front of the pBAD promoter. | + | A construct for controlling the expression of a gene in front of the pBAD promoter. We added the reverse terminator B0025 to the 2014 Imperial part for use in plasmids without a reverse terminator, such as pSB1A2. |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 16:41, 28 October 2016
Arabinose inducible promoter (pBAD)
A construct for controlling the expression of a gene in front of the pBAD promoter. We added the reverse terminator B0025 to the 2014 Imperial part for use in plasmids without a reverse terminator, such as pSB1A2.
Usage and Biology
Without any arabinose in the system, araC forms a homodimer, binding to operator sites upstream of the pBAD promoter, generating an inhibitory loop in the DNA that prevents access by RNAP. In the absence of glucose, and the presence of L-arabinose, the dimer dissociates from the DNA, allowing transcription of whatever is downstream of the pBAD promoter. We have characterised the activation range of this part by using GFP as a reporter construct. You can find the information here (BBa_K1893017).
It is also possible to switch off the pBAD promoter via catabolite repression once the promoter has been induced by L-arabinose by adding D-glucose to the system.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1342
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1281
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1116
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1098