Difference between revisions of "Part:BBa K1976050"
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| − | This part can be used for assembly of generators. The So called miniColicin is an active DNase and In combination with the immunity protein Im2 (part part <a href="https://parts.igem.org/Part:BBa_K197604">BBa_K1976026</a>) it serves as a safety mechanism (see part <a href="https://parts.igem.org/Part:BBa_K197604"> | + | This part can be used for assembly of generators. The So called miniColicin is an active DNase and In combination with the immunity protein Im2 (part part <a href="https://parts.igem.org/Part:BBa_K197604">BBa_K1976026</a>) it serves as a safety mechanism (see part <a href="https://parts.igem.org/Part:BBa_K197604">BBa_K1976052</a>). |
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Revision as of 03:19, 28 October 2016
Colicin E2 DNase domain with B0034 RBS
The colicin DNase domain BBa_K1976048 is fusioned to the strong ribosomal binding site RBSBBa_B0034.
Figure 1:Colicin E2 DNase domain based on an MD simulation.
Usage
This part can be used for assembly of generators. The So called miniColicin is an active DNase and In combination with the immunity protein Im2 (part part BBa_K1976026) it serves as a safety mechanism (see part BBa_K1976052).
Characteristics
The DNase fragment of colicin E2 has a molecular weight of 15.2 kDa and consists of 133 residues what conforms to about one third to colicin. We used only one of the three domains of colicin since the metabolic burdon would be significant smaller. The activity tests showed that the so called miniColicin has functional DNase activity.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 46
- 1000COMPATIBLE WITH RFC[1000]
[1] Cascales et al, Colicin Biology, Microbiology and Molecular Biology Reviews, vol. 71, pp. 158-229, 2007