Difference between revisions of "Part:BBa K2123201"
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This composite part was developed to turn available two <i>mer</i> operon enzymes, related to Hg metabolism: organomercurial lyase (MerB) and mercury reductase (MerA), as you can see below.  
 
This composite part was developed to turn available two <i>mer</i> operon enzymes, related to Hg metabolism: organomercurial lyase (MerB) and mercury reductase (MerA), as you can see below.  
  
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<center><img="https://parts.igem.org/File:UFAM_MERBA_1.png"=></center>
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<center>https://static.igem.org/mediawiki/parts/d/dc/UFAM_MERBA_1.png</center>
 
   
 
   
 
With this part, you can switch: I) promoter sequences, regulating (or not) by what you want; II) transporters protein, using the preferential one for your chassis; III) and whatever you want related to mercury metabolism! These two enzymes together increase the mercury metabolism spectrum in bacteria, improving bioremediation process. Check how the enzymatic pathway works on “Structure and mechanism” and it’s results in “Usage, Methodology and Experiments”.  
 
With this part, you can switch: I) promoter sequences, regulating (or not) by what you want; II) transporters protein, using the preferential one for your chassis; III) and whatever you want related to mercury metabolism! These two enzymes together increase the mercury metabolism spectrum in bacteria, improving bioremediation process. Check how the enzymatic pathway works on “Structure and mechanism” and it’s results in “Usage, Methodology and Experiments”.  

Revision as of 19:49, 27 October 2016


Strong RBS + MerB (Organomercurial Lyase) + Strong RBS + MerA (Mercuric Reductase) + B0015

Overview

This composite part was developed to turn available two mer operon enzymes, related to Hg metabolism: organomercurial lyase (MerB) and mercury reductase (MerA), as you can see below.

UFAM_MERBA_1.png

With this part, you can switch: I) promoter sequences, regulating (or not) by what you want; II) transporters protein, using the preferential one for your chassis; III) and whatever you want related to mercury metabolism! These two enzymes together increase the mercury metabolism spectrum in bacteria, improving bioremediation process. Check how the enzymatic pathway works on “Structure and mechanism” and it’s results in “Usage, Methodology and Experiments”.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 458
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 56
    Illegal NgoMIV site found at 630
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 49