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===Applications of BBa_K1976000===
 
===Applications of BBa_K1976000===
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used for genomic integration with the help of BBa_K1976001
  
 
===User Reviews===
 
===User Reviews===
 
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Enter the review inofrmation here.
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We (TU_Darmstadt 2015) transposed our Biobrick onto a low copy vector (pSB4A5), because it is easier for plasmid curing afterwards. The sequencing results of the transformation into <i>E.&#8239;coli</i>&nbsp;JM109 showed in four cases four different DNA sequences. After a protein blast (using NCBI BLAST) we found a transposase between prefix and suffix of our vector. We assume, that the homologous region in the &lambda;-attP site is recognized by the transposase and it uses the similar sequences of prefix and suffix to translocate between these sites.
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        <img style="width: 95%; height: 140%; margin-left: 15px; margin-right: 15px;" alt="" src="https://static.igem.org/mediawiki/parts/c/ce/T--TU_Darmstadt--Blast_Transposase.png">
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            <b>Figure 1:</b> Blast of our sequencing results showing the ISAs1 family transposase (we expected BBa_K1976000 and got this) (NCBI blastx was used for this)
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Latest revision as of 16:41, 27 October 2016


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1976000

used for genomic integration with the help of BBa_K1976001

User Reviews

UNIQ4079a02386f2a2ec-partinfo-00000000-QINU

No review score entered. TSD

We (TU_Darmstadt 2015) transposed our Biobrick onto a low copy vector (pSB4A5), because it is easier for plasmid curing afterwards. The sequencing results of the transformation into E. coli JM109 showed in four cases four different DNA sequences. After a protein blast (using NCBI BLAST) we found a transposase between prefix and suffix of our vector. We assume, that the homologous region in the λ-attP site is recognized by the transposase and it uses the similar sequences of prefix and suffix to translocate between these sites.

Figure 1: Blast of our sequencing results showing the ISAs1 family transposase (we expected BBa_K1976000 and got this) (NCBI blastx was used for this)

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UNIQ4079a02386f2a2ec-partinfo-00000003-QINU