Difference between revisions of "Part:BBa K1893002:Design"
(→Source) |
|||
| Line 12: | Line 12: | ||
===Source=== | ===Source=== | ||
| − | |||
| − | |||
===References=== | ===References=== | ||
Latest revision as of 14:49, 27 October 2016
Rhl receiver (RhlR+pRhl)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 301
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 776
Design Notes
This part does not include an LVA tag on the RhlR gene. This means that the protein is not rapidly degraded. When active, the RhlR protein binds to the pRhl promoter, activating transcription of downstream genes. This part contains everything necessary for C4-HSL-induced expression of genes inserted downstream of the pRhl promoter.