Difference between revisions of "Part:BBa K1893000:Experience"
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| + | ===Applications of BBa_K1893000=== | ||
We used this part to engineer a bacterial communication system which was bidirectional, so that two different strains could grow together. Each strain in our system must have a distinct HSL associated with it, so we required two quorum sensing systems which were orthogonal. We found that Las and Rhl had very little crosstalk, indicating that they were good candidates for our system. | We used this part to engineer a bacterial communication system which was bidirectional, so that two different strains could grow together. Each strain in our system must have a distinct HSL associated with it, so we required two quorum sensing systems which were orthogonal. We found that Las and Rhl had very little crosstalk, indicating that they were good candidates for our system. | ||
| − | This part worked as expected, and when we placed GFP downstream of the LasR promoter, we saw fluorescence when we induced with 3O-C12 AHL. | + | This part worked as expected, and when we placed GFP downstream of the LasR promoter, we saw fluorescence when we induced with 3O-C12 AHL. |
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===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 13:53, 27 October 2016
Applications of BBa_K1893000
We used this part to engineer a bacterial communication system which was bidirectional, so that two different strains could grow together. Each strain in our system must have a distinct HSL associated with it, so we required two quorum sensing systems which were orthogonal. We found that Las and Rhl had very little crosstalk, indicating that they were good candidates for our system.
This part worked as expected, and when we placed GFP downstream of the LasR promoter, we saw fluorescence when we induced with 3O-C12 AHL.
User Reviews
UNIQ4fe22cadcddd340d-partinfo-00000000-QINU UNIQ4fe22cadcddd340d-partinfo-00000001-QINU