Difference between revisions of "Part:BBa K209472"

 
 
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<partinfo>BBa_K209472 short</partinfo>
 
<partinfo>BBa_K209472 short</partinfo>
  
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This is the coding sequence of Homo sapiens adrenoceptor beta 2 (ADRB2). This part has been improved by SJTU-Biox-Shanghai 2016 iGEM team to generate two parts with modified sequence: BBa_K1969001 and BBa_K1969002.
  
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BBa_K1969001
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Group: SJTU-Biox-Shanghai 2016
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Author: Yijie Lai
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Summary: This improved version using site  mutation to eliminate the illegal PstI site by changing G at 192 bp to A, and was tested for epinephrine response when expressed in yeast Saccharomyces cerevisiae under two different promoters, GAL1 and ADH1.
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BBa_K1969002
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Group: SJTU-Biox-Shanghai 2016
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Author: Yijie Lai
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Summary: This improved version using site  mutation to eliminate the illegal PstI site by changing G at 192 bp to A and adding a his-tag to its C terminus. The sequence was tested for membrane localization when expressed in yeast Saccharomyces cerevisiae under two different promoters, GAL1 and ADH1.
  
  

Latest revision as of 05:13, 27 October 2016


AarI B-D part, ADRA2A (B2AR)

This is the coding sequence of Homo sapiens adrenoceptor beta 2 (ADRB2). This part has been improved by SJTU-Biox-Shanghai 2016 iGEM team to generate two parts with modified sequence: BBa_K1969001 and BBa_K1969002.

BBa_K1969001 Group: SJTU-Biox-Shanghai 2016 Author: Yijie Lai Summary: This improved version using site mutation to eliminate the illegal PstI site by changing G at 192 bp to A, and was tested for epinephrine response when expressed in yeast Saccharomyces cerevisiae under two different promoters, GAL1 and ADH1.

BBa_K1969002 Group: SJTU-Biox-Shanghai 2016 Author: Yijie Lai Summary: This improved version using site mutation to eliminate the illegal PstI site by changing G at 192 bp to A and adding a his-tag to its C terminus. The sequence was tested for membrane localization when expressed in yeast Saccharomyces cerevisiae under two different promoters, GAL1 and ADH1.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 190
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 190
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 778
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 190
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 190
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 364
    Illegal BsaI.rc site found at 562