Difference between revisions of "Part:BBa K239006:Experience"

Latest revision as of 03:27, 27 October 2016

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K239006

User Reviews

UNIQa433355e8cd26836-partinfo-00000000-QINU UNIQa433355e8cd26836-partinfo-00000001-QINU

Please see experience of BBa_K239011 (gives information for usage of this promoter in a devise expressing GFP).

No review score entered. Rice iGEM 2016

For the 2016 Team Rice project, we wanted to build a biosensor in E. coli with 2009 UCL London's mNarK promoter upstream of one of our photoacoustic contrast agents, so that fluorescence would increase in anaerobic conditions. Because of time constraints, we were only able to obtain characterization data for the construct that contains mNarK fused to iRFP670. Interestingly, our experiment indicated that the purging of oxygen was not effective in inducing transcription as originally expected.

Fig 1. Results of part assay (construct output iRFP670) in aerobic, and anaerobic conditions -- oxygen was purged with nitrogen gas. Results are averaged over 8 samples that ran simultaneously in a single experiment.

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 <I>Rice iGEM 2016</I>
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-For the 2016 Team Rice project, we wanted to build a biosensor in <i>E. coli</i> with 2009 UCL London's mNarK promoter upstream of one of our photoacoustic contrast agents, so that fluorescence would increase in anaerobic conditions. Because of time constraints, we were only able to obtain characterization data for the construct that contains mNarK fused to iRFP670. Interestingly, our experiment indicated that <em>the purging of oxygen was not effective in inducing transcription as originally expected</em>.
+For the 2016 Team Rice project, we wanted to build a biosensor in <i>E. coli</i> with 2009 UCL London's mNarK promoter upstream of one of our photoacoustic contrast agents, so that <em>fluorescence would increase in anaerobic conditions</em>. Because of time constraints, we were only able to obtain characterization data for the construct that contains mNarK fused to iRFP670. Interestingly, our experiment indicated that <strong><em>the purging of oxygen was not effective in inducing transcription as originally expected</em></strong>.
 [[Image:MNarK_IRFP670_Hypoxia_Assay.jpg|thumb|left|800px|Fig 1. Results of part assay (construct output iRFP670) in aerobic, and anaerobic conditions -- oxygen was purged with nitrogen gas. Results are averaged over 8 samples that ran simultaneously in a single experiment.]]
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