Difference between revisions of "Part:BBa K2043009"
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| − | The bpul gene (<a href="https://parts.igem.org/Part:BBa_K2043007">Bba_K2043007</a>) codon optimized for <i>E. coli</i> was improved by addition of fabric binding domain | + | The bpul gene (<a href="https://parts.igem.org/Part:BBa_K2043007">Bba_K2043007</a>) codon optimized for <i>E. coli</i> was improved by addition of fabric binding domain 10 (FBD1) on the 3'-end of the sequence using Golden Gate assembly method. FBD10 is the first of 40 short peptides with affinity for cotton, linen, wool, polyester or silk identified in the <a href="http://2016.igem.org/Team:Paris_Bettencourt">Frank&Stain</a> project. FBD10 is reported to have binding affinity for cotton (you can read more on this in the paper by. Unfortunately, this BioBrick was not tested for its activity due to a lack of time in iGEM competition. However, <a href="https://parts.igem.org/Part:BBa_K2043007">bpul</a> and <a href="https://parts.igem.org/Part:BBa_K2043008">FBD1</a> specifications can be found on their BioBrick pages |
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Revision as of 22:56, 26 October 2016
bpuI-FBD10 laccase codon optimized for E.coli
Description
The bpul gene (Bba_K2043007) codon optimized for E. coli was improved by addition of fabric binding domain 10 (FBD1) on the 3'-end of the sequence using Golden Gate assembly method. FBD10 is the first of 40 short peptides with affinity for cotton, linen, wool, polyester or silk identified in the Frank&Stain project. FBD10 is reported to have binding affinity for cotton (you can read more on this in the paper by. Unfortunately, this BioBrick was not tested for its activity due to a lack of time in iGEM competition. However, bpul and FBD1 specifications can be found on their BioBrick pages
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]