Difference between revisions of "Part:BBa K2043009"
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<partinfo>BBa_K2043009 short</partinfo>
 
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bpuI-FBD10 codon optimized from Bacilus pumillus
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<h2>Description</h2>
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The bpul gene (<a href="https://parts.igem.org/Part:BBa_K2043007">Bba_K2043007</a>) codon optimized for <i>E. coli</i> was improved by addition of fabric binding domain 1 (FBD1) on the 3'-end of the sequence using Golden Gate assembly method. FBD1 is the first of 40 short peptides with affinity for cotton, linen, wool, polyester or silk identified in the <a href="http://2016.igem.org/Team:Paris_Bettencourt">Frank&Stain</a> project. FBD1 is supposed to have binding affinity for cotton. Unfortunately, this BioBrick was not tested for its activity due to a lack of time in iGEM competition. However, <a href="https://parts.igem.org/Part:BBa_K2043007">bpul</a> and <a href="https://parts.igem.org/Part:BBa_K2043008">FBD1</a> specifications can be found on their BioBrick pages
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===Usage and Biology===
 
===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 22:26, 26 October 2016


bpuI-FBD10 laccase codon optimized for E.coli


Description

The bpul gene (Bba_K2043007) codon optimized for E. coli was improved by addition of fabric binding domain 1 (FBD1) on the 3'-end of the sequence using Golden Gate assembly method. FBD1 is the first of 40 short peptides with affinity for cotton, linen, wool, polyester or silk identified in the Frank&Stain project. FBD1 is supposed to have binding affinity for cotton. Unfortunately, this BioBrick was not tested for its activity due to a lack of time in iGEM competition. However, bpul and FBD1 specifications can be found on their BioBrick pages


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


bpuI - FBD10