Difference between revisions of "Part:BBa K2116004"

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<partinfo>BBa_K2116004 short</partinfo>
 
<partinfo>BBa_K2116004 short</partinfo>
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<p>
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We constructed a selection of AND gates responding to nitric oxide (NO) and 3OC6HSL (AHL). They were designed using the previously described NorV promoter [[Part:BBa_K1153000]].
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This Promoter (from here on refered to as PnorV) is the native promoter controlling the nitric oxide reduction operon (norRVW) in <i>E. coli</i> [1]. Its transcriptional regulator,
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NorR, can bind to nitric oxide and activate gene expression.
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Using the distinct properties of esaboxes, PnorV was designed to also be responsive to AHL, giving it an AND gate behaviour.
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An esabox is an 18bp sequence to which the transcriptional regulator EsaR [[Part:BBa_K2116001]] can bind. Transcription can be initiated by the specific AHL EsaR responds
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to [N-(3-oxo-hexanoyl)-L-homoserine lactone]. By placing one, two or three esaboxes at different positions in the vicinity of PnorV, different specificities for AHL and NO
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were reached. We created and characterized a collection of these kind of AND gates: <br>
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<ul>
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<li> [[Part:BBa_K2116004]]
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<li> [[Part:BBa_K2116005]]
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<li> [[Part:BBa_K2116006]]
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<li> [[Part:BBa_K2116012]]
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<li> [[Part:BBa_K2116013]]
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<li> [[Part:BBa_K2116014]]
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<li> [[Part:BBa_K2116007]]
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<li> [[Part:BBa_K2116008]]
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<li> [[Part:BBa_K2116068]]
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<li> [[Part:BBa_K2116015]]
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</ul>
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</p>
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<h2> Characterization of this part </h2>
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Data will be here before the final presentation at the jamboree. Apologies for the delay.
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<h2>References:</h2>
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<ul>
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<li> [1] Gardner, A. M. "Regulation Of The Nitric Oxide Reduction Operon (Norrvw) In Escherichia Coli. ROLE OF Norr AND Sigma 54 IN THE NITRIC OXIDE STRESS RESPONSE". Journal of Biological Chemistry 278.12 (2003): 10081-10086.</i>
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<li> [2] Shong, Jasmine and Cynthia H. Collins. "Engineering The Esar Promoter For Tunable Quorum Sensing-Dependent Gene Expression". ACS Synth. Biol. 2.10 (2013): 568-575. </li>
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This AND gate was constructed using PnorV [[Part:BBa_K1153000]] and one esabox, positioned after the transcription start site to serve as a roadblock.
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 14:33, 26 October 2016


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For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.

We constructed a selection of AND gates responding to nitric oxide (NO) and 3OC6HSL (AHL). They were designed using the previously described NorV promoter Part:BBa_K1153000. This Promoter (from here on refered to as PnorV) is the native promoter controlling the nitric oxide reduction operon (norRVW) in E. coli [1]. Its transcriptional regulator, NorR, can bind to nitric oxide and activate gene expression. Using the distinct properties of esaboxes, PnorV was designed to also be responsive to AHL, giving it an AND gate behaviour. An esabox is an 18bp sequence to which the transcriptional regulator EsaR Part:BBa_K2116001 can bind. Transcription can be initiated by the specific AHL EsaR responds to [N-(3-oxo-hexanoyl)-L-homoserine lactone]. By placing one, two or three esaboxes at different positions in the vicinity of PnorV, different specificities for AHL and NO were reached. We created and characterized a collection of these kind of AND gates:


Characterization of this part

Data will be here before the final presentation at the jamboree. Apologies for the delay.

References:


  • [1] Gardner, A. M. "Regulation Of The Nitric Oxide Reduction Operon (Norrvw) In Escherichia Coli. ROLE OF Norr AND Sigma 54 IN THE NITRIC OXIDE STRESS RESPONSE". Journal of Biological Chemistry 278.12 (2003): 10081-10086.</i>
  • [2] Shong, Jasmine and Cynthia H. Collins. "Engineering The Esar Promoter For Tunable Quorum Sensing-Dependent Gene Expression". ACS Synth. Biol. 2.10 (2013): 568-575.


    • Sequence and Features Status: 500 Content-type: text/html

    Software error:

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Global symbol "$groups" requires explicit package name at /websites/parts.igem.org/cgi/lib/User.pm line 85.
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    For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.