Difference between revisions of "Part:BBa K2116015"
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AND gate constructed by adding an esabox before the -24 on the sigma 54 binding site of PnorV. This esabox overlaps the sigma 54 binding site, creating competitive binding between the polymerase and EsaR. | AND gate constructed by adding an esabox before the -24 on the sigma 54 binding site of PnorV. This esabox overlaps the sigma 54 binding site, creating competitive binding between the polymerase and EsaR. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
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Revision as of 14:18, 26 October 2016
PnorV_esabox_(15bp)spacer_esabox_spacer_esabox (p30)
AND gate constructed by adding an esabox before the -24 on the sigma 54 binding site of PnorV. This esabox overlaps the sigma 54 binding site, creating competitive binding between the polymerase and EsaR.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]