Difference between revisions of "Part:BBa K1878004:Experience"

Latest revision as of 07:33, 26 October 2016

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Applications of BBa_K1878004

User Reviews

UNIQ131899b9b4f0d597-partinfo-00000000-QINU UNIQ131899b9b4f0d597-partinfo-00000001-QINU

No review score entered. Rice iGEM 2016

For the 2016 Team Rice project, we built a biosensor construct in E. coli with fdhF and iRFP670 to fluoresce in the absence of oxygen. Specifically, the cofactor fdhA binds to formate, a metabolite in anaerobic respiration, to upregulate transcription downstream of fdhF. However, despite this description from literature, our assay results indicate that transcription was downregulated in anaerobic conditions.

Fig 1. Results of part assay in aerobic, and anaerobic, conditions.

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+{|width='80%' style='border:1px solid gray'
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+<partinfo>BBa_K1878004 AddReview number</partinfo>
+<I>Rice iGEM 2016</I>
+|width='60%' valign='top'|
+For the 2016 Team Rice project, we built a biosensor construct in <i>E. coli</i> with fdhF and iRFP670 to fluoresce in the absence of oxygen. Specifically, the cofactor fdhA binds to formate, a metabolite in anaerobic respiration, to upregulate transcription downstream of fdhF. However, despite this description from literature, our assay results indicate that transcription was downregulated in anaerobic conditions.
+[[Image:Fdhf_assay_670.jpg|thumb|left|800px|Fig 1. Results of part assay in aerobic, and anaerobic, conditions.]]
+|}