Difference between revisions of "Part:BBa K1878001:Experience"

 
 
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<I>Rice iGEM 2016</I>
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For the 2016 Team Rice project, we used the fluorescent protein iRFP713 in <i>E. coli</i> for proof-of-concept experiments for our biosensors -- our future experiments would include genes encoding the enzymes to produce the small-molecule pigment Violacein, instead of fluorescent proteins. We placed the promoter pBAD upstream for protein production during these characterization experiments.
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[[Image:IRFP713_Excitation_Spectrum.jpg|thumb|left|800px|Fig 1. Results of part characterization for excitation wavelengths. Production was induced under promoter pBAD, with 2mM arabinose]]
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[[Image:IRFP713_Emission_Spectrum.jpg|thumb|left|800px|Fig 2. Results of part characterization for emission wavelengths. Production was induced under promoter pBAD, with 2mM arabinose]]
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Latest revision as of 07:01, 26 October 2016


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Applications of BBa_K1878001

User Reviews

UNIQ9e5568cd4e3a00ce-partinfo-00000000-QINU UNIQ9e5568cd4e3a00ce-partinfo-00000001-QINU

No review score entered. Rice iGEM 2016

For the 2016 Team Rice project, we used the fluorescent protein iRFP713 in E. coli for proof-of-concept experiments for our biosensors -- our future experiments would include genes encoding the enzymes to produce the small-molecule pigment Violacein, instead of fluorescent proteins. We placed the promoter pBAD upstream for protein production during these characterization experiments.

Fig 1. Results of part characterization for excitation wavelengths. Production was induced under promoter pBAD, with 2mM arabinose
Fig 2. Results of part characterization for emission wavelengths. Production was induced under promoter pBAD, with 2mM arabinose