Difference between revisions of "Part:BBa K1985016"
 
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<partinfo>BBa_K1985016 short</partinfo>
 
<partinfo>BBa_K1985016 short</partinfo>
  
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This part encodes Part:BBa_K1321333 in a pSB1A3 backbone. It was ligated to this backbone as it confers the ampicillin resistance that was needed for in vivo expression for our composite parts: Bba_K1985017, Bba_K1985008, Bba_K1985009, Bba_K1985013, Bba_K1985014, Bba_K1985015.
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This part encodes (Part:[https://parts.igem.org/Part:BBa_K1321333 BBa_K1321333]) in a pSB1A3 backbone. It was ligated to this backbone as it confers the ampicillin resistance that was needed to express our composite parts: [https://parts.igem.org/Part:BBa_K1985017 BBa_K1985017], [https://parts.igem.org/Part:BBa_K1985008 BBa_K1985008], [https://parts.igem.org/Part:BBa_K1985009 BBa_K1985009], [https://parts.igem.org/Part:BBa_K1985013 BBa_K1985013], [https://parts.igem.org/Part:BBa_K1985014 BBa_K1985014] and [https://parts.igem.org/Part:BBa_K1985015 BBa_K1985015].
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1985016 SequenceAndFeatures</partinfo>
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===Validation===
 
===Validation===
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[[File:PSB1A3+promoter.jpeg||400px|thumb|centre|Figure 1.1% agarose gel of the restriction digest of BBa_K1985016 in pSB1A3 plasmid backbone with EcoRI and PstI]]
 
[[File:PSB1A3+promoter.jpeg||400px|thumb|centre|Figure 1.1% agarose gel of the restriction digest of BBa_K1985016 in pSB1A3 plasmid backbone with EcoRI and PstI]]
  
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<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_K1985016 SequenceAndFeatures</partinfo>
 
  
  

Latest revision as of 18:18, 25 October 2016

AraC-pBAD in pSB1A3

This part encodes (Part:BBa_K1321333) in a pSB1A3 backbone. It was ligated to this backbone as it confers the ampicillin resistance that was needed to express our composite parts: BBa_K1985017, BBa_K1985008, BBa_K1985009, BBa_K1985013, BBa_K1985014 and BBa_K1985015.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Validation

The plasmid was analysed through a diagnostic double restriction cut, using the enzymes EcoRI and PStI. This was followed by agarose gel electrophoresis. The enzymes cleave the pBAD Ara-C promoter at 1165 bp, with the remainder plasmid being 2114 bp. The sizes of the two fragments were compared with the size of the Invitrogen 1kB DNA marker and was found that our fragments were the correct.

Figure 1.1% agarose gel of the restriction digest of BBa_K1985016 in pSB1A3 plasmid backbone with EcoRI and PstI