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	<h1>Colicin E2 DNase trypsin fragment and immunity protein Im2 generator with anderson promoters</h1>		<h1>Colicin E2 DNase trypsin fragment and immunity protein Im2 generator with anderson promoters</h1>
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−	<a href="https://parts.igem.org/Part:BBa_K197604">BBa_K1976049</a> and <a href="https://parts.igem.org/Part:BBa_K1976026">BBa_K1976026</a>	+	Generator of colicin E2 DNase trypsin fragment <a href="https://parts.igem.org/Part:BBa_K197604">BBa_K1976049</a> and immunity protein Im2 <a href="https://parts.igem.org/Part:BBa_K1976026">BBa_K1976026</a>.
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Revision as of 14:19, 25 October 2016

Colicin E2 DNase trypsin fragment and immunity protein Im2 generator with anderson promoters

Generator of colicin E2 DNase trypsin fragment BBa_K1976049 and immunity protein Im2 BBa_K1976026.

Usage

This part can be used to produce colicin without killing the host cell, as the immunity protein is produced more than equimolar to the DNase fragment.

Characteristics

Molecular Weight	Colicin E2 Dnase domain 15.2 kDa Immunity Protein 10.0 kDa
Residues	Colicin E2 Dnase domain 133
pI	Colicin E2 Dnase domain 9.06 Immunity Protein 4.66
[Data taken created in Snapgene and http://isoelectric.ovh.org/calculate.php]

Characteristics

Sequence and Features

[1] Cascales et al, Colicin Biology, Microbiology and Molecular Biology Reviews, vol. 71, pp. 158-229, 2007