Difference between revisions of "Part:BBa K1976000"
Line 5: Line 5:
 
===Usage and Biology===
 
===Usage and Biology===
  
−
<p> Green fluorescent protein with attachment-site-plasmid (attP) of phage &lambda;. By genomic integration of this part into bacteria carrying the bacterial attachment site (attB), the metabolic burden on the microorganism can be quantified measuring the GFP concentration. The genomic integration is achieved by attP/attB recombination and catalysed by the &lambda;-integrase. <b>Further information will follow in the next days (Nov./22/2016)</b></p>
+
<p> Green fluorescent protein with attachment-site-plasmid (attP) of phage &lambda;. By genomic integration of this part into bacteria carrying the bacterial attachment site (attB), the metabolic burden on the microorganism can be quantified measuring the GFP concentration. The genomic integration is achieved by attP/attB recombination and catalysed by the &lambda;-integrase.</p>
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 10:48, 25 October 2016


Integration plasmid

Usage and Biology

Green fluorescent protein with attachment-site-plasmid (attP) of phage λ. By genomic integration of this part into bacteria carrying the bacterial attachment site (attB), the metabolic burden on the microorganism can be quantified measuring the GFP concentration. The genomic integration is achieved by attP/attB recombination and catalysed by the λ-integrase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 315
    Illegal NheI site found at 338
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1014