Difference between revisions of "Part:BBa K2070005"
 
(One intermediate revision by the same user not shown)
Line 8: Line 8:
  
 
[[File:T--UT-Tokyo--BBa K2070005--3.png|500px]]
 
[[File:T--UT-Tokyo--BBa K2070005--3.png|500px]]
 +
 +
===Usage and Biology===
 +
 +
 +
[[File:T--UT-Tokyo--BBa K2070005-1.png|thumb|centre|700px|'''Measurement of ECF sigma35 and sigma30 promoter activity. '''Fluorescence/OD600 of a construct containing both an ECF sigma35 generating device <partinfo>BBa_K2070013</partinfo> and ECF sigma 35 inducible GFP generating device <partinfo>BBa_K2070005</partinfo> was compared to that containing only either one of the devices. *** for P> 0.001 indicates an extremely significant difference between the induced and uninduced state. ]]
 +
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 07:18, 25 October 2016


Constitutive ecf35 generator with ecf35 inducible GFP reporter

This part, consisting of a constitutive ECF sigma35 generator BBa_K2070013 and ECF sigma35 inducible GFP reporter BBa_K2070004, was used to test the functionality of ECF sigma35 and its cognate promoter. See experience page for details of the result.


T--UT-Tokyo--BBa K2070005--3.png

Usage and Biology

Measurement of ECF sigma35 and sigma30 promoter activity. Fluorescence/OD600 of a construct containing both an ECF sigma35 generating device BBa_K2070013 and ECF sigma 35 inducible GFP generating device BBa_K2070005 was compared to that containing only either one of the devices. *** for P> 0.001 indicates an extremely significant difference between the induced and uninduced state.