Difference between revisions of "Part:BBa K2070005"
 
(2 intermediate revisions by the same user not shown)
Line 5: Line 5:
 
This part, consisting of a constitutive ECF sigma35 generator [https://parts.igem.org/Part:BBa_K2070013 BBa_K2070013] and ECF sigma35 inducible GFP reporter [https://parts.igem.org/Part:BBa_K2070004 BBa_K2070004], was used to test the functionality of ECF sigma35 and its cognate promoter. See experience page for details of the result.  
 
This part, consisting of a constitutive ECF sigma35 generator [https://parts.igem.org/Part:BBa_K2070013 BBa_K2070013] and ECF sigma35 inducible GFP reporter [https://parts.igem.org/Part:BBa_K2070004 BBa_K2070004], was used to test the functionality of ECF sigma35 and its cognate promoter. See experience page for details of the result.  
  
−
<!-- Add more about the biology of this part here
+
 
 +
 
 +
[[File:T--UT-Tokyo--BBa K2070005--3.png|500px]]
 +
 
 
===Usage and Biology===
 
===Usage and Biology===
  
−
<!-- -->
+
 
−
<span class='h3bb'>Sequence and Features</span>
+
[[File:T--UT-Tokyo--BBa K2070005-1.png|thumb|centre|700px|'''Measurement of ECF sigma35 and sigma30 promoter activity. '''Fluorescence/OD600 of a construct containing both an ECF sigma35 generating device <partinfo>BBa_K2070013</partinfo> and ECF sigma 35 inducible GFP generating device <partinfo>BBa_K2070005</partinfo> was compared to that containing only either one of the devices. *** for P> 0.001 indicates an extremely significant difference between the induced and uninduced state. ]]
−
<partinfo>BBa_K2070005 SequenceAndFeatures</partinfo>
+
 
 +
 
 +
<!-- Add more about the biology of this part here
 +
===Usage and Biology===
  
  

Latest revision as of 07:18, 25 October 2016


Constitutive ecf35 generator with ecf35 inducible GFP reporter

This part, consisting of a constitutive ECF sigma35 generator BBa_K2070013 and ECF sigma35 inducible GFP reporter BBa_K2070004, was used to test the functionality of ECF sigma35 and its cognate promoter. See experience page for details of the result.


T--UT-Tokyo--BBa K2070005--3.png

Usage and Biology

Measurement of ECF sigma35 and sigma30 promoter activity. Fluorescence/OD600 of a construct containing both an ECF sigma35 generating device BBa_K2070013 and ECF sigma 35 inducible GFP generating device BBa_K2070005 was compared to that containing only either one of the devices. *** for P> 0.001 indicates an extremely significant difference between the induced and uninduced state.