Difference between revisions of "Part:BBa K2070013"

Latest revision as of 07:03, 25 October 2016

Constitutive ecf35 generator

This part contains an ECF35 coding region downstream of a medium rbs(BBa_B0032) and strong promoter (BBa_J23101). The ecf35 generated can activate the ecf30 promoter (BBa_K2070000).

Usage and Biology

Measurement of ECF sigma35 and sigma30 promoter activity.Fluorescence/OD600 of a construct containing both an ECF sigma35 generating device BBa_K2070013 and ECF sigma 35 inducible GFP generating device BBa_K2070005 was compared to that containing only either one of the devices. *** for P> 0.001 indicates an extremely significant difference between the induced and uninduced state.

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 This part contains an ECF35 coding region downstream of a medium rbs(BBa_B0032) and strong promoter (BBa_J23101). The ecf35 generated can activate the ecf30 promoter (BBa_K2070000).
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+[[File:T--UT-Tokyo--BBa K2070005-1.png|thumb|centre|700px|'''Measurement of ECF sigma35 and sigma30 promoter activity.'''Fluorescence/OD600 of a construct containing both an ECF sigma35 generating device BBa_K2070013 and ECF sigma 35 inducible GFP generating device BBa_K2070005 was compared to that containing only either one of the devices. *** for P> 0.001 indicates an extremely significant difference between the induced and uninduced state.]]
-<!-- -->
-<span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K2070013 SequenceAndFeatures</partinfo>