Difference between revisions of "Part:BBa K2036001"

 
 
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<partinfo>BBa_K2036001 short</partinfo>
 
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inhibitor of Ftsh( ATP-dependent host metalloprotease)
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Inhibitor of Ftsh( ATP-dependent host metalloprotease).
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<br>The CIII protein encoded by the temperate coliphage lambda acts as an inhibitor of the ubiquitous Escherichia coli metalloprotease HflB (FtsH). This inhibition results in the stabilization of transcription factor lambda CII, thereby helping the phage to lysogenize the host bacterium.
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===Usage and Biology===
 
===Usage and Biology===
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<partinfo>BBa_K2036001 parameters</partinfo>
 
<partinfo>BBa_K2036001 parameters</partinfo>
 
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<h1>Protein&protein reaction</h1>
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We had submitted and documented RBS-CIII-RBS-CIII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag ([https://parts.igem.org/Part:BBa_K2036014 BBa_K2036014]) and RBS-CII-RBS-CII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag ([https://parts.igem.org/Part:BBa_K2036015 BBa_K2036015]). These two parts were to test whether CIII can protect CII from being degraded by Ftsh by competitive inhibition.
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[[File:T--HUST-China--CIII%26Ftsh.png|800px|thumb|center|Fig1: According to the flourescence measurement curve above, we can see clearly that GFP level of CIII test circuit increasedover time and it showed significant difference from two control groups. It indicates that tandemly expressed CIII can efficiently protect CII from being degraded by Ftsh.]]

Latest revision as of 06:49, 25 October 2016


CIII

Inhibitor of Ftsh( ATP-dependent host metalloprotease).


The CIII protein encoded by the temperate coliphage lambda acts as an inhibitor of the ubiquitous Escherichia coli metalloprotease HflB (FtsH). This inhibition results in the stabilization of transcription factor lambda CII, thereby helping the phage to lysogenize the host bacterium.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Protein&protein reaction

We had submitted and documented RBS-CIII-RBS-CIII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag (BBa_K2036014) and RBS-CII-RBS-CII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag (BBa_K2036015). These two parts were to test whether CIII can protect CII from being degraded by Ftsh by competitive inhibition.


Fig1: According to the flourescence measurement curve above, we can see clearly that GFP level of CIII test circuit increasedover time and it showed significant difference from two control groups. It indicates that tandemly expressed CIII can efficiently protect CII from being degraded by Ftsh.