Difference between revisions of "Part:BBa K2070020:Experience"

Latest revision as of 06:48, 25 October 2016

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Please enter how you used this part and how it worked out.

[http://2016.igem.org/Team:UT-Tokyo iGEM UT-Tokyo 2016]

Measurement of nrd Promoter Activity. The fluorescence from the Pnrd promoter (ligated with BBa_E0240) is compared with that from the standard promoter BBa_J23101, 3 samples were measured every 9 minutes for each construct. Although there seems to be a slight sudden rise in the promoter activity from the 10-20th minute after the start of measurement, since the change in cell concentration could not be measured simultaneously with fluorescence, it is not certain whether the rise is influenced by the cell cycle/division.

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Applications of BBa_K2070020

User Reviews

UNIQ98ae2beb296a2884-partinfo-00000000-QINU UNIQ98ae2beb296a2884-partinfo-00000001-QINU

@@ Line 3: / Line 3: @@
 This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 how you used this part and how it worked out.
+{|width='80%' style='border:1px solid gray'
+|-
+|width='10%'|
+<I>[http://2016.igem.org/Team:UT-Tokyo iGEM UT-Tokyo 2016]</I>
+|width='60%' valign='top'|
+[[File:T--UT-Tokyo--K2070012.png|thumb|700px|'''Measurement of nrd Promoter Activity.'''
+The fluorescence from the Pnrd promoter (ligated with BBa_E0240) is compared with that from the standard promoter BBa_J23101, 3 samples were measured every 9 minutes for each construct. Although there seems to be a slight sudden rise in the promoter activity from the 10-20th minute after the start of measurement, since the change in cell concentration could not be measured simultaneously with fluorescence, it is not certain whether the rise is influenced by the cell cycle/division.]]
+|};
 ===Applications of BBa_K2070020===