Difference between revisions of "Part:BBa K2036006"

 
 
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<partinfo>BBa_K2036006 short</partinfo>
 
<partinfo>BBa_K2036006 short</partinfo>
  
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CIII is a inhibitor of Ftsh,we add a RBS to provide more possibilities to users to create more complex circuit.
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CIII ( [https://parts.igem.org/Part:BBa_K2036001 BBa_K2036001] )is a inhibitor of FtsH,we add a RBS to provide more possibilities to users to create more complex circuit.
  
 
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<partinfo>BBa_K2036006 parameters</partinfo>
 
<partinfo>BBa_K2036006 parameters</partinfo>
 
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<h1>Protein&protein reaction</h1>
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<p>
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We had submitted and documented RBS-CIII-RBS-CIII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag ([https://parts.igem.org/Part:BBa_K2036014 BBa_K2036014]) and RBS-CII-RBS-CII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag ([https://parts.igem.org/Part:BBa_K2036015 BBa_K2036015]). These two parts were to test whether CIII can protect CII from being degraded by FtsH by competitive inhibition.
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[[File:T--HUST-China--CIII%26Ftsh.png|800px|thumb|center|Fig1: According to the flourescence measurement curve above, we can see clearly that GFP level of CIII test circuit increased over time and it showed significant difference from two control groups. It indicates that tandemly expressed CIII can efficiently protect CII from being degraded by Ftsh.]]

Latest revision as of 06:36, 25 October 2016


RBS-CIII

CIII ( BBa_K2036001 )is a inhibitor of FtsH,we add a RBS to provide more possibilities to users to create more complex circuit.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Protein&protein reaction

We had submitted and documented RBS-CIII-RBS-CIII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag (BBa_K2036014) and RBS-CII-RBS-CII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag (BBa_K2036015). These two parts were to test whether CIII can protect CII from being degraded by FtsH by competitive inhibition.


Fig1: According to the flourescence measurement curve above, we can see clearly that GFP level of CIII test circuit increased over time and it showed significant difference from two control groups. It indicates that tandemly expressed CIII can efficiently protect CII from being degraded by Ftsh.