Difference between revisions of "Part:BBa K2130007"

 
 
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Human codon optimised Acidaminococcus sp. BV3L6 (AsCpf1)
 
Human codon optimised Acidaminococcus sp. BV3L6 (AsCpf1)
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Similar to LbCpf1, it depends on TTTN PAM for target recognition and cleavage. The optimal gRNA length is 19-23 nucleotides. Cpf1 is proven to achieve more specific cleavage activity compared to Cas9 endonclease.
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<html><center><img src="https://static.igem.org/mediawiki/parts/b/be/AsCpf1.PNG" height="60%"width="75%">
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<img src="https://static.igem.org/mediawiki/2016/f/fc/T--NTU-Singapore--bigtest.jpg" height="60%"width="75%"></center>
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<img src="https://static.igem.org/mediawiki/2016/e/e2/T--NTU-Singapore--CDStest.jpg" height="60%"width="75%"></center>
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<img src=" https://static.igem.org/mediawiki/2016/b/bb/T--NTU-Singapore--seed.jpg" height="60%"width="75%"></center>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 13:56, 24 October 2016


AsCpf1

Human codon optimised Acidaminococcus sp. BV3L6 (AsCpf1)

Similar to LbCpf1, it depends on TTTN PAM for target recognition and cleavage. The optimal gRNA length is 19-23 nucleotides. Cpf1 is proven to achieve more specific cleavage activity compared to Cas9 endonclease.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 25
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 407
    Illegal BglII site found at 1949
    Illegal BglII site found at 2258
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3493
    Illegal NgoMIV site found at 3954
    Illegal NgoMIV site found at 3973
  • 1000
    COMPATIBLE WITH RFC[1000]