Difference between revisions of "Part:BBa K892008:Experience"
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| − | This biobrick part was used to assemble a device that contains a rhamnose-responsive promoter <partinfo>BBa_K914003</partinfo>, a strong RBS | + | This biobrick part was used to assemble a device that contains a rhamnose-responsive promoter <partinfo>BBa_K914003</partinfo>, a strong RBS <partinfo>BBa_B0030</partinfo>) and OsmY amplified with a C-terminal HA tag resulting in the following composite part <partinfo>BBa_K1963007</partinfo>. The device showed secretion of OsmY under rhamnose-regulated conditions. |
| − | We also used this OsmY biobrick to create two translational fusion proteins (Hfq from E. coli <partinfo> BBa_K1963005</partinfo> and Serratia <partinfo>BBa_K1963006</partinfo>). These two biobricks were then amplified with a C-terminal HA tag and hooked up to the <i>rhamnose</i> promoter in order test for rhamnose-regulated expression of an sRNA secretion system | + | We also used this OsmY biobrick to create two translational fusion proteins (Hfq from E. coli <partinfo>BBa_K1963005</partinfo> and Serratia <partinfo>BBa_K1963006</partinfo>). These two biobricks were then amplified with a C-terminal HA tag and hooked up to the <i>rhamnose</i> promoter in order test for rhamnose-regulated expression of an sRNA secretion system |
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Latest revision as of 12:24, 24 October 2016
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K892008
User Reviews
UNIQcf379f5429f598d8-partinfo-00000000-QINU
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iGEM Dundee 2016 |
This biobrick part was used to assemble a device that contains a rhamnose-responsive promoter BBa_K914003, a strong RBS BBa_B0030) and OsmY amplified with a C-terminal HA tag resulting in the following composite part BBa_K1963007. The device showed secretion of OsmY under rhamnose-regulated conditions. We also used this OsmY biobrick to create two translational fusion proteins (Hfq from E. coli BBa_K1963005 and Serratia BBa_K1963006). These two biobricks were then amplified with a C-terminal HA tag and hooked up to the rhamnose promoter in order test for rhamnose-regulated expression of an sRNA secretion system |
User Reviews
UNIQcf379f5429f598d8-partinfo-00000007-QINU UNIQcf379f5429f598d8-partinfo-00000008-QINU