Difference between revisions of "Part:BBa K2130005"

Latest revision as of 12:20, 24 October 2016

NmCas9

Human codon optimised NmCas9

NmCas9 is originated from Neisseria meningitidis species with a PAM requirement of NGGNGATT that placed at the 3' end of spacer sequence. From the results attached, it shows that NmCas9 is generally not performed well for both NHEJ and HDR even with different spacer length that range from 17-23 nucleotides.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 511
Illegal NgoMIV site found at 3282
Illegal NgoMIV site found at 3301
Illegal AgeI site found at 3271
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1373
Illegal BsaI.rc site found at 2557

@@ Line 5: / Line 5: @@
 Human codon optimised NmCas9
-NmCas9 is originated from Neisseria meningitidis species with a PAM requirement of NGGNGATT that placed at 3' end of spacer sequence. From the results attached, it shows that NmCas9 is generally not performed well for both NHEJ and HDR even with different spacer length that range from 17-23 nucleotides.
+NmCas9 is originated from Neisseria meningitidis species with a PAM requirement of NGGNGATT that placed at the 3' end of spacer sequence. From the results attached, it shows that NmCas9 is generally not performed well for both NHEJ and HDR even with different spacer length that range from 17-23 nucleotides.
-<html><center><img src="https://static.igem.org/mediawiki/2016/b/b4/T--NTU-Singapore--evaconstructs.jpg" height="60%"width="75%">
+<html><center><img src="https://static.igem.org/mediawiki/parts/0/0b/NmCas9.PNG" height="60%"width="75%">
 <img src="https://static.igem.org/mediawiki/2016/f/fc/T--NTU-Singapore--bigtest.jpg" height="60%"width="75%"></center>