Difference between revisions of "Part:BBa K2043005:Design"
Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
− | + | His-tag added for enhanced purification. <br> | |
+ | Gene was codon optimised for expression in <i>E. coli</i> | ||
Revision as of 12:08, 24 October 2016
xylE-FBD1 from Pseudomonas putida codon optimized for E. coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 700
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
His-tag added for enhanced purification.
Gene was codon optimised for expression in E. coli
Source
Pseudomonas putida