|
|
| Line 19: |
Line 19: |
| | <!-- End of the user review template --> | | <!-- End of the user review template --> |
| | <!-- DON'T DELETE --><partinfo>BBa_K2120421 EndReviews</partinfo> | | <!-- DON'T DELETE --><partinfo>BBa_K2120421 EndReviews</partinfo> |
| − | {|width='80%' style='border:1px solid gray'
| |
| − | |-
| |
| − | |width='10%'|
| |
| − | <partinfo>BBa_K2120421 AddReview 5</partinfo>
| |
| − | <I><B>[http://2016.igem.org/Team:BIT-China BIT-China iGEM 2016 Team]</B></I>
| |
| − | |width='60%' valign='top'|
| |
| − | <br>We have linked this circuit in pSB1K3, pSB3K3 and pSB4K5 and tested them in ''E.coli BMTop10''. We cultured our bacteria overnight and then tested fluorescence intensity to indicate the expression level of circuit in different plasmids.
| |
| − | <br>The fluorescence intensity is shown in Fig.1. As,we all know,pSB1K3 has the highest copy number and the copy number of pSB3K3 is little higher than the copy number of pSB4K5. And according to the result, the fluorescence intensity of bacteria containing pSB4K5 is higher than containing pSB3K3. The fluorescence intensity of bacteria containing pSB1K3 is the lowest. It means our system is working. Our killer would express more with less plasmids.
| |
| − | [[File:BIT-CHINA-PARTS-INHIBITOR-23.jpg|thumb|Fig 1. The fluorescence intensity expressed by different bacteria containing different combine with constitutive promoter and plasmids.|600px|center]]
| |
| − | |};
| |
Latest revision as of 17:23, 23 October 2016
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K2120421
User Reviews
UNIQ6bdae5b7b88fccac-partinfo-00000000-QINU
UNIQ6bdae5b7b88fccac-partinfo-00000001-QINU
