Difference between revisions of "Part:BBa K1976049"

 
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        <h1>Colicin E2 immunity protein</h1>
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Colicin E2 is a peptide, that is toxic to coliform bacteria. It is one of a group of proteins of this type, it being a nicking endonuclease. It has a corresponding activity inhibiting protein called immunity protein Im2, which is also in the registry under the name .
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<partinfo>BBa_K1976049 short</partinfo>
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<b>Figure 1:</b>Structure of Colicin E2 DNase domain derived from a molecular dynamics simulation. <b>Note:</b> this is not actually this part but <a href="https://parts.igem.org/Part:BBa_K1976048">BBa_K1976048</a>, a 5 amino acids shorter version
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The fragment of Colicin, which one would obtain from treating Colicin E2 with Trypsin
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===<h2>Usage</h2>===
  
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===Usage and Biology===
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The Colicin E2 immunity protein (<a href="https://parts.igem.org/Part:BBa_K1976027">BBa_K1976027</a> and <a href="https://parts.igem.org/Part:BBa_K1976028">BBa_K1976028</a>) can be used in combination with the Colicin E2 DNase.
  
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<span class='h3bb'>Sequence and Features</span>
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===<h2>Characteristics</h2>===
  
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<h2>Sequence and Features</h2>
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===Functional Parameters===
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<partinfo>BBa_K1976049 parameters</partinfo>
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<b>References</b>
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[1] Cascales et al, Colicin Biology, Microbiology and Molecular Biology Reviews, vol. 71, pp. 158-229, 2007<br>
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Revision as of 16:59, 23 October 2016

Colicin E2 immunity protein

Colicin E2 is a peptide, that is toxic to coliform bacteria. It is one of a group of proteins of this type, it being a nicking endonuclease. It has a corresponding activity inhibiting protein called immunity protein Im2, which is also in the registry under the name .

Figure 1:Structure of Colicin E2 DNase domain derived from a molecular dynamics simulation. Note: this is not actually this part but BBa_K1976048, a 5 amino acids shorter version


Usage

The Colicin E2 immunity protein (BBa_K1976027 and BBa_K1976028) can be used in combination with the Colicin E2 DNase.

Characteristics

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 46
  • 1000
    COMPATIBLE WITH RFC[1000]


References
[1] Cascales et al, Colicin Biology, Microbiology and Molecular Biology Reviews, vol. 71, pp. 158-229, 2007