Difference between revisions of "Part:BBa K2180011"

Revision as of 15:43, 23 October 2016

Arsenic Operator - ArsR Binding Site

Biobrick based on a previous biobrick made by the iGEM09_Newcastle Team (2009-10-14) :
https://parts.igem.org/Part:BBa_K174015
We added two restriction sites: SacII before and PacI after the regulatory region in order to use it in our project. They allow us to replace a regulatory region with another regulatory region. This Region allows ArsR to bind to DNA and repress transcription. In the presence of Heavy Metals such as Arsenic or Cadmium transcription is derepressed because of the fixation to ArsR.
Other metalic ions may bind to ArsR.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2180011 short</partinfo>
-We add two restriction sites. SacII before and PacI after the regulatory region in order to use it in our project. They allow us to replace a regulatory region with another regulatory region. This Region allow ArsR to bind to DNA and repress transcription. In the presence of Heavy Metals such as Arsenic or Cadmium transcription is derepressed because of the fixation to ArsR.
+Biobrick based on a previous biobrick made by the iGEM09_Newcastle Team (2009-10-14) :
-Other metalic ions may bind to ArsR.
+<br>https://parts.igem.org/Part:BBa_K174015
+<br>We added two restriction sites: SacII before and PacI after the regulatory region in order to use it in our project. They allow us to replace a regulatory region with another regulatory region. This Region allows ArsR to bind to DNA and repress transcription. In the presence of Heavy Metals such as Arsenic or Cadmium transcription is derepressed because of the fixation to ArsR.
+<br>Other metalic ions may bind to ArsR.
 <!-- Add more about the biology of this part here