Difference between revisions of "Part:BBa K2180009"

 
 
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<partinfo>BBa_K2180009 short</partinfo>
 
<partinfo>BBa_K2180009 short</partinfo>
  
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This is the assembly of our basic parts BBa_K2180004 , BBa_K2180007 without the Fur Box and BBa_K2180008
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The sequence is based on a previous IGEM part made by the group Antiquity (2003-01-31) coding for LacI + LVA for E. coli:
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We delete the Fur Box in order to use it like a normal promoter.
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<br>https://parts.igem.org/Part:BBa_C0012
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The NcoI restriction site is overlapping parts BBa_K2180004 and BBa_K2180007 at the Start codon of LacI + LVA.
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<br>This biobrick is the coding sequence of LacI protein + LVA (rapid degradation tail) wich improve the switch time and make it more sensitive.
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<br>We had to improve the codon usage for B. subtilis
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 15:39, 23 October 2016


LacI-LVA for B. subtilis

The sequence is based on a previous IGEM part made by the group Antiquity (2003-01-31) coding for LacI + LVA for E. coli:
https://parts.igem.org/Part:BBa_C0012
This biobrick is the coding sequence of LacI protein + LVA (rapid degradation tail) wich improve the switch time and make it more sensitive.
We had to improve the codon usage for B. subtilis

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 178
    Illegal AgeI site found at 966
  • 1000
    COMPATIBLE WITH RFC[1000]