Difference between revisions of "Part:BBa K2180002"

 
 
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<partinfo>BBa_K2180002 short</partinfo>
 
<partinfo>BBa_K2180002 short</partinfo>
  
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We add two restriction sites. SacII before and PacI after the regulatory region in order to use it in our project. They allow us to replace a regulatory region with another regulatory region.
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The sequence of the regulatory region comes from this site
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The regulatory region is composed by two FruR binding sites wich are derepressed in the presence of fructose.
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<br>We added two restriction sites: SacII before and PacI after the regulatory region in order to use it in our project.
 +
<br>This restriction sites allow us to replace a regulatory region with another regulatory region.
 +
<br>The regulatory region is composed by two FruR binding sites wich are derepressed in the presence of fructose.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 15:26, 23 October 2016


Fructose Operator - FruR Binding Site

The sequence of the regulatory region comes from this site
We added two restriction sites: SacII before and PacI after the regulatory region in order to use it in our project.
This restriction sites allow us to replace a regulatory region with another regulatory region.
The regulatory region is composed by two FruR binding sites wich are derepressed in the presence of fructose.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]