Difference between revisions of "Part:BBa K1893015"

Revision as of 15:21, 23 October 2016

Arabinose inducible promoter (pBAD)

A construct for controlling the expression of a gene in front of the pBAD promoter.

Usage and Biology

Without any arabinose in the system, araC forms a homodimer, binding to operator sites upstream of the pBAD promoter, generating an inhibitory loop in the DNA that prevents access by RNAP. In the absence of glucose, and the presence of L-arabinose, the dimer dissociates from the DNA, allowing transcription of whatever is downstream of the pBAD promoter. We have characterised the activation range of this part by using GFP as a reporter construct. You can find the information here.

It is also possible to switch off the pBAD promoter via catabolite repression once the promoter has been induced by L-arabinose by adding D-glucose to the system.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1342
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1281
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1116
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 1098

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 ===Usage and Biology===
-Without any arabinose in the system, araC forms a homodimer, binding to operator sites upstream of the pBAD promoter, generating an inhibitory loop in the DNA that prevents access by RNAP. In the absence of glucose, and the presence of L-arabinose, the dimer dissociates from the DNA, allowing transcription of whatever is downstream of the pBAD promoter. We have characterised the activation range of this part by using GFP as a reporter construct. You can find the information here.
+Without any arabinose in the system, araC forms a homodimer, binding to operator sites upstream of the pBAD promoter, generating an inhibitory loop in the DNA that prevents access by RNAP. In the absence of glucose, and the presence of L-arabinose, the dimer dissociates from the DNA, allowing transcription of whatever is downstream of the pBAD promoter. We have characterised the activation range of this part by using GFP as a reporter construct. You can find the information [https://parts.igem.org/Part:BBa_K1893017 here].
 It is also possible to switch off the pBAD promoter via catabolite repression once the promoter has been induced by L-arabinose by adding D-glucose to the system.