Difference between revisions of "Part:BBa C0077"
Line 8: Line 8:
  
 
In ''Rhizobium leguminosarum'', cinR and cinI are forward-pointing, adjacent, and separate transcriptional elements. There is a short (<200bp) intergenic region between cinR and cinI. The cinR/intergenic/cinI structure is conserved for the cinRI locus in ''Rhizobium etli'', and for the cerRI locus in ''Rhodobacter sphaeroides''. The intergenic region is thought to contain a terminator for cinR, a promoter and RBS for cinI, and a dna-binding domain for cinR and its homologs, though a conserved structure has not yet been identified.
 
In ''Rhizobium leguminosarum'', cinR and cinI are forward-pointing, adjacent, and separate transcriptional elements. There is a short (<200bp) intergenic region between cinR and cinI. The cinR/intergenic/cinI structure is conserved for the cinRI locus in ''Rhizobium etli'', and for the cerRI locus in ''Rhodobacter sphaeroides''. The intergenic region is thought to contain a terminator for cinR, a promoter and RBS for cinI, and a dna-binding domain for cinR and its homologs, though a conserved structure has not yet been identified.
 +
 +
====I. CinR (no LVA) Part by Imperial College London iGEM 2016 ====
 +
 +
Group: <b>Imperial College London 2016</b>
 +
 +
We created a new version of this part without the LVA degradation tag [https://parts.igem.org/Part:BBa_K1893028 (BBa_K1893028)].
 +
 +
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 14:06, 23 October 2016

cinR activator from Rhizobium leguminosarum (+LVA)

In complex with O3-C14:1-HSL (made by BBa_C0076), CinR binds to the Cin promoter (BBa_R0077) and activates transcription.


Usage and Biology

In Rhizobium leguminosarum, cinR and cinI are forward-pointing, adjacent, and separate transcriptional elements. There is a short (<200bp) intergenic region between cinR and cinI. The cinR/intergenic/cinI structure is conserved for the cinRI locus in Rhizobium etli, and for the cerRI locus in Rhodobacter sphaeroides. The intergenic region is thought to contain a terminator for cinR, a promoter and RBS for cinI, and a dna-binding domain for cinR and its homologs, though a conserved structure has not yet been identified.

I. CinR (no LVA) Part by Imperial College London iGEM 2016

Group: Imperial College London 2016

We created a new version of this part without the LVA degradation tag (BBa_K1893028).


Sequence and Features


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 528
  • 1000
    COMPATIBLE WITH RFC[1000]