Difference between revisions of "Part:BBa K1954002"

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Production of Vitellogenin-GFP fusion under pGAP promoter.
  
  
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<partinfo>BBa_K1954002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1954002 SequenceAndFeatures</partinfo>
  
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===Usage and Biology===
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The protein Vitellogenin has previously been expressed in the methyl-trophic yeast <i>Pichia pastoris</i> which has shown to impact cellular production of essential amino acids and long-chain polyunsaturated fatty acids. This effect is consistent with the putative role of Vitellogenin in the health of metazoans, a group which includes wasps (<i>Encarsia formosa</i>) and Silverleaf whitefly (<i>Bemisia tabaci</i>). Targeting Vitellogenin for increased production in certain wasps, or decreased production in Silverleaf whitefly, could both be future routes to controlling the whitefly as a pest.
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 14:04, 23 October 2016


pGAP-Vitellogenin-GFP device

Production of Vitellogenin-GFP fusion under pGAP promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1905
    Illegal NheI site found at 2139
    Illegal NheI site found at 4457
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
    Illegal BglII site found at 3274
    Illegal BglII site found at 3900
    Illegal BglII site found at 3936
    Illegal BamHI site found at 1281
    Illegal XhoI site found at 577
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 806
    Illegal BsaI site found at 3681
    Illegal BsaI site found at 3811
    Illegal SapI site found at 244
    Illegal SapI site found at 3868
    Illegal SapI.rc site found at 5732


Usage and Biology

The protein Vitellogenin has previously been expressed in the methyl-trophic yeast Pichia pastoris which has shown to impact cellular production of essential amino acids and long-chain polyunsaturated fatty acids. This effect is consistent with the putative role of Vitellogenin in the health of metazoans, a group which includes wasps (Encarsia formosa) and Silverleaf whitefly (Bemisia tabaci). Targeting Vitellogenin for increased production in certain wasps, or decreased production in Silverleaf whitefly, could both be future routes to controlling the whitefly as a pest.