Difference between revisions of "Part:BBa K2120413"

Latest revision as of 12:44, 23 October 2016

B0015+cI+B0034+J23116+pR+B0032+mazF+B0015

<Description>
This is one of our application circuits.

This circuit contains two devices: inhibitor device and killer device.
Inhibitor device: We select medium weak constitutive promoter J23116 and strong RBS B0034 to express inhibitor gene cI.
Killer device: Toxin protein, MazF, can cleavage ACA sequence of free mRNA specifically. It cause bacteria can’t translate successfully and dead finally. So we constructed it with induced promoter pR and medium RBS B0032 to control the expression level of toxin protein.
We reversed the direction of inhibitor device, in order to reduce the influence between two devices.
Meanwhile, we also constructed more circuits which have different combine with promoter, RBS and inhibitor. More detail see in our Wiki([http://2016.igem.org/Team:BIT-China/Parts Parts Submission])

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 941
Illegal NheI site found at 964
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
+'''<Description>'''
+<br>This is one of our application circuits.
+<br>This circuit contains two devices: inhibitor device and killer device.
+<br>Inhibitor device: We select medium weak constitutive promoter J23116 and strong RBS B0034 to express inhibitor gene ''cI''.
+<br>Killer device: Toxin protein, MazF, can cleavage ACA sequence of free mRNA specifically. It cause bacteria can’t translate successfully and dead finally. So we constructed it with induced promoter pR and medium RBS B0032 to control the expression level of toxin protein.
+<br>We reversed the direction of inhibitor device, in order to reduce the influence between two devices.
+<br>Meanwhile, we also constructed more circuits which have different combine with promoter, RBS and inhibitor. More detail see in our Wiki([http://2016.igem.org/Team:BIT-China/Parts Parts Submission])