Difference between revisions of "Part:BBa K2120403"
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<partinfo>BBa_K2120403 short</partinfo> | <partinfo>BBa_K2120403 short</partinfo> | ||
− | This is one of our application | + | '''<Description>''' |
+ | <br>This is one of our application circuits. | ||
− | + | <br>This circuit contains two devices: inhibitor device and killer device. | |
− | + | <br>Inhibitor device: We select weak constitutive promoter J23109 and strong RBS B0034 to express inhibitor gene ''tetR''. | |
− | We | + | <br>Killer device: Toxin protein, MazF, can cleavage ACA sequence of free mRNA specifically. It cause bacteria can’t translate successfully and dead finally. So we constructed it with induced promoter pTet and strong RBS B0034 to control the expression level of toxin protein. |
− | + | <br>We reversed the direction of inhibitor device, in order to reduce the influence between two devices. | |
− | + | <br>Meanwhile, we also constructed more circuits which have different combine with promoter, RBS and inhibitor. More details see in our Wiki([http://2016.igem.org/Team:BIT-China/Parts Parts Submission]) | |
Latest revision as of 12:27, 23 October 2016
B0015+tetR+B0034+J23109+ptet+B0034+mazF+B0015
<Description>
This is one of our application circuits.
This circuit contains two devices: inhibitor device and killer device.
Inhibitor device: We select weak constitutive promoter J23109 and strong RBS B0034 to express inhibitor gene tetR.
Killer device: Toxin protein, MazF, can cleavage ACA sequence of free mRNA specifically. It cause bacteria can’t translate successfully and dead finally. So we constructed it with induced promoter pTet and strong RBS B0034 to control the expression level of toxin protein.
We reversed the direction of inhibitor device, in order to reduce the influence between two devices.
Meanwhile, we also constructed more circuits which have different combine with promoter, RBS and inhibitor. More details see in our Wiki([http://2016.igem.org/Team:BIT-China/Parts Parts Submission])
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 851
Illegal NheI site found at 874 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]