Difference between revisions of "Part:BBa K1893013:Design"
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===Design Notes===
 
===Design Notes===
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This sequence was synthesised as a block with BioBrick Prefix on the 5' end and BioBrick Suffix on the 3' end.
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This sequence was obtained through the use of inverse PCR on the 'j23119-STAR/pSB1C3' construct (BBa_K1893010) to remove the constitutive promoter j23119.  
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 +
STAR Forward primer:
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TGAACTGTATACATTCCCCGC
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Prefix Reverse primer:
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CTCTAGAAGCGGCCGCG
  
 
===Source===
 
===Source===
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===References===
 
===References===
  
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Meyer, S., Chappell, J., Sankar, S., Chew, R. and Lucks, J. (2015). Improving fold activation of small transcription activating RNAs (STARs) with rational RNA engineering strategies. Biotechnol. Bioeng., 113(1), pp.216-225.
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Chappell, J., Takahashi, M. & Lucks, J. (2015) Creating small transcription activating RNAs. Nature Chemical Biology. [Online] 11 (3), 214-220

Revision as of 12:00, 23 October 2016


Small transcription activating RNA (STAR)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence was obtained through the use of inverse PCR on the 'j23119-STAR/pSB1C3' construct (BBa_K1893010) to remove the constitutive promoter j23119.

STAR Forward primer: TGAACTGTATACATTCCCCGC

Prefix Reverse primer: CTCTAGAAGCGGCCGCG

Source

The pAD1.A5 STAR sequence (STAR-antisense)

References

Chappell, J., Takahashi, M. & Lucks, J. (2015) Creating small transcription activating RNAs. Nature Chemical Biology. [Online] 11 (3), 214-220