Difference between revisions of "Part:BBa K1893009:Design"
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<partinfo>BBa_K1893009 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1893009 SequenceAndFeatures</partinfo> | ||
| − | + | ===Design Notes=== | |
| − | This sequence was synthesised as a block. | + | This sequence was synthesised as a block with BioBrick Prefix on the 5' end and BioBrick Suffix on the 3' end. |
===Source=== | ===Source=== | ||
Latest revision as of 11:11, 23 October 2016
STAR inducible superfolder GFP (pAD1.S5+SF-GFP)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 569
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 959
Illegal BsaI site found at 1137
Design Notes
This sequence was synthesised as a block with BioBrick Prefix on the 5' end and BioBrick Suffix on the 3' end.
Source
The pAD1.S5 attenuator sequence (STAR target)
References
Chappell, J., Takahashi, M. & Lucks, J. (2015) Creating small transcription activating RNAs. Nature Chemical Biology. [Online] 11 (3), 214-220