Difference between revisions of "Part:BBa K2120420"

Latest revision as of 08:43, 23 October 2016

B0015+tetR+B0034+J23106+ptet+B0034+mRFP+B0015

This is one of our application circuit.

<Description>
This device is similar to our project final gene circuit using relationship of inhibitor-promoter to start downstream gene’s transcription.We use RFP as a report gene. The report gene could indicate a safety condition when killer gene express.
In order to adjust the repressing efficiency, we choose medium strong constitutive promoter J23106 and strong RBS B0034 to express inhibitor gene tetR. Meanwhile, we also constructed more circuits which have different combine with promoter, RBS and inhibitor. More detail see in our Wiki([http://2016.igem.org/Team:BIT-China/Parts Parts Submission])

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 851
Illegal NheI site found at 874
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1528
Illegal AgeI site found at 1640
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2120420 short</partinfo>
+This is one of our application circuit.
+'''<Description>'''
+<br>This device is similar to our project final gene circuit using relationship of inhibitor-promoter to start downstream gene’s transcription.We use ''RFP'' as a report gene. The report gene could indicate a safety condition when killer gene express.
+<br>In order to adjust the repressing efficiency, we choose medium strong constitutive promoter J23106 and strong RBS B0034 to express inhibitor gene ''tetR''. Meanwhile, we also constructed more circuits which have different combine with promoter, RBS and inhibitor. More detail see in our Wiki([http://2016.igem.org/Team:BIT-China/Parts Parts Submission])