Difference between revisions of "Part:BBa K2120416"
 
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This is one of our application circuit.
 
This is one of our application circuit.
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This circuit consists of two parts:the inhibitor device and reporter device ,which have already been made into basic parts of biobricks.
 
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We choose three different promoters (BBa_J23109,BBa_J23116,BBa_J23106) whose reported strengths are respectively low, medium and strong from constitutive promoter family to express the inhibitor tetR and cI ,
 
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a reporter protein-mRFP (BBa_E1010) was used as a signal to represent if the concentration of inhibitor controlled by different promoters can repress the expression of downstream gene controllled by inducible promoter completely .At first,we test fluorescence intensity of mRFP protein expressed by each circuit in high copy plasmid pSB1K3 ,the results show that inhibitor devices with J23116 and J23109 cannot inhibit the expression of mRFP ,which means that the strength of these two promoters is too weak to turn off the reporter device in our circuit.
 
  
  
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'''<Description>'''
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<br>This device is similar to our project final gene circuit using relationship of inhibitor-promoter to start downstream gene’s transcription.We use ''RFP'' as a report gene. The report gene could indicate a safety condition when killer gene express.
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<br>In order to adjust the repressing efficiency, we choose strong constitutive promoter J23119 and strong RBS B0034 to express inhibitor gene ''tetR''. Meanwhile, we also constructed more circuits which have different combine with promoter, RBS and inhibitor. More detail see in our Wiki([http://2016.igem.org/Team:BIT-China/Parts Parts Submission])
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 08:30, 23 October 2016


B0015+tetR+B0034+J23119+pTet+B0034+mRFP+B0015

This is one of our application circuit.


<Description>
This device is similar to our project final gene circuit using relationship of inhibitor-promoter to start downstream gene’s transcription.We use RFP as a report gene. The report gene could indicate a safety condition when killer gene express.
In order to adjust the repressing efficiency, we choose strong constitutive promoter J23119 and strong RBS B0034 to express inhibitor gene tetR. Meanwhile, we also constructed more circuits which have different combine with promoter, RBS and inhibitor. More detail see in our Wiki([http://2016.igem.org/Team:BIT-China/Parts Parts Submission])

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 851
    Illegal NheI site found at 874
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1528
    Illegal AgeI site found at 1640
  • 1000
    COMPATIBLE WITH RFC[1000]