Difference between revisions of "Part:BBa K1961005:Design"
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===Design Notes=== | ===Design Notes=== | ||
PbrR is a Pb2+ binding protein. In addition to the PbrR generator with the PTet promoter(K1961004), we designed a PbrR generator (K1961005; also with GFP reporter) under control of general promoter(J23102). We compared their GFP expression to measure their PbrR generation. | PbrR is a Pb2+ binding protein. In addition to the PbrR generator with the PTet promoter(K1961004), we designed a PbrR generator (K1961005; also with GFP reporter) under control of general promoter(J23102). We compared their GFP expression to measure their PbrR generation. | ||
− | + | [[File: HSiTAIWAN_WIKI_K1961005_map_1.jpeg|500px|thumb|left|alt text]] | |
===Source=== | ===Source=== | ||
− | We used parts from iGEM stocks | + | We used parts from iGEM stocks: J23102+B0032+I721002+B0010+B0012+I721001+B0034+E1010+B0010+B0012 |
===References=== | ===References=== |
Latest revision as of 07:23, 23 October 2016
PbrR generator expressed by GFP under control of general promoter (J23102)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1111
Design Notes
PbrR is a Pb2+ binding protein. In addition to the PbrR generator with the PTet promoter(K1961004), we designed a PbrR generator (K1961005; also with GFP reporter) under control of general promoter(J23102). We compared their GFP expression to measure their PbrR generation.
Source
We used parts from iGEM stocks: J23102+B0032+I721002+B0010+B0012+I721001+B0034+E1010+B0010+B0012