Difference between revisions of "Part:BBa K1961004:Design"

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===Design Notes===
 
===Design Notes===
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This sequence consists of two sub-parts: (1) TetR generator under under control of PTet, which has TetR binding sites; (2) PbrR generator expressed by GFP under control of PTet.  We used tetracycline to induce the transcription.
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This sequence consists of two sub-parts: (1) TetR generator under under control of PTet, which has TetR binding sites; (2) PbrR generator expressed by GFP under control of PTet.  We used tetracycline to induce the transcription. In order to transcribe PbrR and GFP, we removed the stop codon at PbrR
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[[File:[[File:File.png|400px|thumb|left|alt text]] |200px|thumb|left|alt text]]
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[[File: HSiTAIWAN_WIKI_K1961004_auto_map.png|400px|thumb|left|alt text]]
  
 
===Source===
 
===Source===

Latest revision as of 07:14, 23 October 2016


PbrR generator expressed by GFP under autoregulation of PTet


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2040


Design Notes

This sequence consists of two sub-parts: (1) TetR generator under under control of PTet, which has TetR binding sites; (2) PbrR generator expressed by GFP under control of PTet. We used tetracycline to induce the transcription. In order to transcribe PbrR and GFP, we removed the stop codon at PbrR


alt text

Source

We use parts in the 2016 iGEM plate R0040, B0034, C0040, B0010, B0012, R0040, B0034, I721002, E0040, B0010, B0012

References