Difference between revisions of "Part:BBa K1963009"
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| − | Usage and Biology | + | ===Usage and Biology=== |
| + | Experimental procedures here. | ||
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| + | https://static.igem.org/mediawiki/parts/7/73/Hfq-serratia-frulhuq4.png | ||
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| + | Figure 1. Whole cell blots expressing OsmY-Hfq fusion protein under the control of the rhamnose promoter. | ||
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| + | https://static.igem.org/mediawiki/parts/8/81/Allsupernatant_blots2.png | ||
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| + | Figure 2. Supernatant blots of OsmY-HA (<partinfo>BBa_K1963007</partinfo>), OsmY-Hfq (E. coli- <partinfo>BBa_K1963008</partinfo>) and OsmY-Hfq (Serratia <partinfo>BBa_K1963009</partinfo>).. Western blotting with anti-HA antibody shows secretion of OsmY-HA, and OsmY-Hfq (E. coli) and anti-RNAP antibody shows no antigen detected in the supernatant therefore the cells remained intact and there was no cell lysis. | ||
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Latest revision as of 22:44, 22 October 2016
Device for rhamnose-dependent expression of an OsmY fusion to Serratia Hfq.
A rhamnose-regulated device based on existing and new (2016) biobricks. This is designed to induce production of a fusion protein between E. coli OsmY (a secerted protein) and Serratia marcescens Hfq (an RNA binding protein). Co-expression with Serratia sRNA may generate a specific RNA secretion system.
Usage and Biology
Experimental procedures here.
Figure 1. Whole cell blots expressing OsmY-Hfq fusion protein under the control of the rhamnose promoter.
Figure 2. Supernatant blots of OsmY-HA (BBa_K1963007), OsmY-Hfq (E. coli- BBa_K1963008) and OsmY-Hfq (Serratia BBa_K1963009).. Western blotting with anti-HA antibody shows secretion of OsmY-HA, and OsmY-Hfq (E. coli) and anti-RNAP antibody shows no antigen detected in the supernatant therefore the cells remained intact and there was no cell lysis.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]