Difference between revisions of "Part:BBa K1963008"
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| − | Figure 2. Supernatant blots of OsmY-HA, OsmY-Hfq (E. coli) and OsmY-Hfq (Serratia). Western blotting with anti-HA antibody shows secretion of OsmY-HA, and OsmY-Hfq (E. coli) and anti-RNAP antibody shows no antigen detected in the supernatant therefore the cells remained intact and there was no cell lysis. | + | Figure 2. Supernatant blots of OsmY-HA (<partinfo>BBa_K1963007</partinfo>), OsmY-Hfq (E. coli- <partinfo>BBa_K1963008</partinfo>) and OsmY-Hfq (Serratia <partinfo>BBa_K1963009</partinfo>). Western blotting with anti-HA antibody shows secretion of OsmY-HA, and OsmY-Hfq (E. coli) and anti-RNAP antibody shows no antigen detected in the supernatant therefore the cells remained intact and there was no cell lysis. |
Latest revision as of 22:42, 22 October 2016
Device for rhamnose-dependent expression of an OsmY fusion to E. coli Hfq..
A composite of three biobricks to give rhamnose-regulated expression of an sRNA secretion system - a translational fusion between E. coli OsmY and E. coli Hfq.
Usage and Biology
Experimental procedures needed.
Figure 1. Whole cell blots expressing OsmY-Hfq (E.coli) fusion protein under the control of the rhamnose promoter.
Figure 2. Supernatant blots of OsmY-HA (BBa_K1963007), OsmY-Hfq (E. coli- BBa_K1963008) and OsmY-Hfq (Serratia BBa_K1963009). Western blotting with anti-HA antibody shows secretion of OsmY-HA, and OsmY-Hfq (E. coli) and anti-RNAP antibody shows no antigen detected in the supernatant therefore the cells remained intact and there was no cell lysis.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]