Difference between revisions of "Part:BBa K1963008"

 
(3 intermediate revisions by the same user not shown)
Line 7: Line 7:
  
 
===Usage and Biology===
 
===Usage and Biology===
 +
 +
 +
Experimental procedures needed.
 +
 +
  
  
Line 16: Line 21:
 
https://static.igem.org/mediawiki/parts/8/81/Allsupernatant_blots2.png
 
https://static.igem.org/mediawiki/parts/8/81/Allsupernatant_blots2.png
  
Figure 2. Supernatant blots of OsmY-HA, OsmY-Hfq (E. coli) and OsmY-Hfq (Serratia). Western blotting with anti-HA antibody shows secretion of OsmY-HA, and OsmY-Hfq (E. coli) and anti-RNAP antibody shows no antigen detected in the supernatant therefore the cells remained intact and there was no cell lysis.  
+
Figure 2. Supernatant blots of OsmY-HA (<partinfo>BBa_K1963007</partinfo>), OsmY-Hfq (E. coli- <partinfo>BBa_K1963008</partinfo>) and OsmY-Hfq (Serratia <partinfo>BBa_K1963009</partinfo>). Western blotting with anti-HA antibody shows secretion of OsmY-HA, and OsmY-Hfq (E. coli) and anti-RNAP antibody shows no antigen detected in the supernatant therefore the cells remained intact and there was no cell lysis.  
  
  

Latest revision as of 22:42, 22 October 2016


Device for rhamnose-dependent expression of an OsmY fusion to E. coli Hfq..

A composite of three biobricks to give rhamnose-regulated expression of an sRNA secretion system - a translational fusion between E. coli OsmY and E. coli Hfq.


Usage and Biology

Experimental procedures needed.



Hfq-serratia-frulhuq5.png

Figure 1. Whole cell blots expressing OsmY-Hfq (E.coli) fusion protein under the control of the rhamnose promoter.


Allsupernatant_blots2.png

Figure 2. Supernatant blots of OsmY-HA (BBa_K1963007), OsmY-Hfq (E. coli- BBa_K1963008) and OsmY-Hfq (Serratia BBa_K1963009). Western blotting with anti-HA antibody shows secretion of OsmY-HA, and OsmY-Hfq (E. coli) and anti-RNAP antibody shows no antigen detected in the supernatant therefore the cells remained intact and there was no cell lysis.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]