Difference between revisions of "Part:BBa K1963004"
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This is a multi-sequence system for small RNA (sRNA) production in an <i>E. coli</i> host - or especially with co-expression of <i>E. coli</i> Hfq <partinfo>BBa_K1963000</partinfo>. The system is based on the <partinfo>BBa_K1963002</partinfo> template and has the <i>proD</i> strong promoter, followed by a short antisense sequence targeting the 5' end of the <i>fliC</i> transcript, followed by the <i>E. coli micC</i> hairpin, followed by the strong terminator sequence T1/TE. The anti <i>fliC</i> sRNA is placed betweeen C-193 and T-194. The system should impair motility of <i>E. coli</i>.
 
This is a multi-sequence system for small RNA (sRNA) production in an <i>E. coli</i> host - or especially with co-expression of <i>E. coli</i> Hfq <partinfo>BBa_K1963000</partinfo>. The system is based on the <partinfo>BBa_K1963002</partinfo> template and has the <i>proD</i> strong promoter, followed by a short antisense sequence targeting the 5' end of the <i>fliC</i> transcript, followed by the <i>E. coli micC</i> hairpin, followed by the strong terminator sequence T1/TE. The anti <i>fliC</i> sRNA is placed betweeen C-193 and T-194. The system should impair motility of <i>E. coli</i>.
  
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Usage and Biology
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===Usage and Biology===
  
  

Revision as of 22:08, 22 October 2016


An sRNA system for down-regulation of E. coli fliC encoding flagellin.

This is a multi-sequence system for small RNA (sRNA) production in an E. coli host - or especially with co-expression of E. coli Hfq BBa_K1963000. The system is based on the BBa_K1963002 template and has the proD strong promoter, followed by a short antisense sequence targeting the 5' end of the fliC transcript, followed by the E. coli micC hairpin, followed by the strong terminator sequence T1/TE. The anti fliC sRNA is placed betweeen C-193 and T-194. The system should impair motility of E. coli.

Usage and Biology

Ecoliflicfrulhuq2.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 101